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美国Hausser细胞计数板
美国Hausser细胞计数板（Nageotte Counting Chamber）：亮线型，常用于单采血小板中的残留白细胞的计数。池深：0.5mm。提供两种：一种H型，中间有沟隔开为两计数区（货号4000），一种没有沟槽（4001）美国Hausser scientific血球计数板，霍华德霉菌计数板，准确计算白细胞、红细胞、血小板、组织培养细胞、细菌和精子The Hausser Nageotte Bright-Line Hemacytometer allows one to manually count low leukocyte concentrations below the capabilities of a standard Hemacytometer. The Nageotte contains two gridded counting areas each containing 40 rectangular areas. Total volume of each grid is 50 mL for a total volume of 100 mL per Nageotte. The Nageotte can accuray measure down to 0.1 cell/mL (100 cell/ml). Cell Depth is 0.5mm .
美国Hausser细胞计数板
ailable with an H-shaped moat(catalog#4000) that separates the two gridded areas or without a moat (catalog#4001). The Nageotte Chamber comes with two reusable cover slips(30mm X 33mm X 0.5mm) and 1/2 oz (approx. 35 pcs.) of disposable cover slips (30mm X 33mm X 0.15mm). PROCESS: Counting low levels of white cells in white cell-reduced plaetcomponents.BACKGROUND: Determination of the white cell (WBC) count in WBC-reduced plaet components requires methods that he a detection limit in the range of approximay 5.0 x 10(2) to 5.0 x 10(4) per mL.STUDY DESIGN AND METHODS: With a 50-microL Nageotte hemocytometer and bright-field microscopy (200x magnification), studies were conducted to develop and validate a method that could be used routinely with filtered and apheresis-harvested plaets. A 1-in-5 dilution of sample with a commercially ailable blood-diluting fluid was used because, with a lower (1-in-2) dilution, the observed number of WBCs was substantially less than the number pected at relatively high plaet counts (> 1.9 x 10(9)/mL). RESULTS: The observed and pected WBC counts in WBC-reduced plaet samples correlated well at levels between approximay 5 and 1100 WBCs per counting area (5.0 x 10(2)-1.1 x 10(5)/mL). At levels of more than 300 to 400 WBCs per counting area, accurate counts were obtained when 10 of the 40 rectangles were counted.
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